The Oscar winner, 63, posted a shot Wednesday of two bags of plasma, along with two other images documenting the process.
“Plasmatic on 3! 1,2,3 PLASMATIC! Hanx,” he wrote.
Just a month ago, Hanks posted about his first plasma donation, calling it “as easy as taking a nap.”
Hanks and wife, singer Rita Wilson, also 63, vowed after recovering from coronavirus that they would both donate.
“We have not only been approached, we have said, ‘Do you want our blood? Can we give plasma?’ And, in fact, we will be giving it now to the places that hope to work on what I would like to call the Hank-ccine,” Hanks said on NPR’s podcast “Wait Wait…Don’t Tell Me!”
Hanks said the couple learned that they had antibodies after their bout with COVID-19, which they were diagnosed with in March in Australia while Hanks was working on Baz Luhrmann’s now-delayed Elvis Presley biopic.
Hanks has said that Wilson had a much “tougher time” battling the illness than himself.
“She had a much higher fever and she had some other symptoms,” he said. “She lost her sense of taste and smell. She got absolutely no joy from food for a better part of three weeks.”