The tests were conducted on Thursday and Friday this week.
They are the first to take place since top-flight clubs agreed on a return to contact training.
A total of 12 people tested positive from over 2,700 tests conducted in the first three rounds.
“The Premier League can today confirm that on Thursday 28 May and Friday 29 May, 1130 players and club staff were tested for COVID-19. Of these, zero have tested positive.
“The Premier League is providing this aggregated information for the purposes of competition integrity and transparency. No specific details as to clubs or individuals will be provided by the League and results will be made public after each round of testing,” a Premier League statement read.
A provisional date of Premier League football return has been fixed for June 17.